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석 사
  Characterization, secretory production, and utility as a reporter gene of   recombinant exoinulinase expressed in Saccharomyces cerevisiae
박 사

  Development of versatile technology for manipulation of large size DNA in   Saccharomyces cerevisiae(Adviser; Dr. Satoshi Harashima, Osaka   university)

 
     
   
 


 - Japan Society for Bioscience, Biotechnology, and Agrochemistry (2002 ~)

 - The Molecular Biology Society of Japan (2001 ~)

 - Yeast Genetics Society of Japan (2003 ~)

 - The Society of Biotechnology of Japan (2003 ~)

 - 한국미생물생명공학회 (2007 ~)

 - 한국생물공학회 (2007 ~)

 
     
   
 


 1. Nam, S.W., Shin, D.H. and Kim, Y.H. (1997). Effect of culture conditions on expression and
   secretion of inulinase and invertase in recombinant Saccharomyces cerevisiae. Kor. J. Appl.
   Microbiol. Biotechnol.,25(3) :258-265.

 2. Nam, S.W., Kim, H.W. Kim, Y.H. and Kim, B.W. (1998). Purification and characterization of
   Kluyveromyces marxianusinulinase from recombinant Saccharomyces cerevisiae. Food Sci. Biotechnol., 7(1): 41-45.

 3. Kim, Y.H., Nam, S.W. and Chung, B.H. (1998). Simultaneous saccharification of inulin and ethanol
   fermentation by recombinant Saccharomyces cerevisiae secreting inulinase. Biotechnol.
Bioprocess Eng., 3(2): 55-60.

 4. Kim, Y.H. and Nam, S.W. (1999). Expression of inulinase gene by yeast constitutive promoters.
   Korean J. Life Science., 9(2): 153-159.

 5. Kim, Y.K., Ko. J.H., Kim, Y.H., Kim, S.K. and Nam, S.W. (2001). Selection of constitutive promoter
   for exoinulinase production in fed-batch culture of recombinant yeast. Kor. J. Appl. Microbiol.
   Biotechnol., 29(4): 206-211.

 6. Liu, H., Kawabe, A., Matsunaga, S., Kim, Y.H., Higashi, T., Uchiyama, S., Harashima, S.,
   Kobayashi, A. and Fukui K. (2004) An Arabidopsis thaliana gene on the yeast artificial
   chromosome can be transcribed in tobacco cells. Cytologia, 69(2): 235-240.

 7. Sugiyama, M., Ikushima, S., Nakazawa, T., Kim, Y.H., Yamagishi, K., Kaneko, Y. and Harashima,
   S.(2004) 효모게놈의 대규모 변환기술의 개발과 바이오사이언스, 바이오테크놀로지에의 응용, 일본생물공학회지
    (ISSN 0919-3758), 82권, 11호, 538-543.

 8. Kim, Y.H., Kaneko, Y., Fukui, K., Kobayashi, A., Harashima, S. (2005) A yeast artificial
   chromosome-splitting vector designed for precise manipulation of specific plant chromosome
   region. J. Biosc. Bioeng., 99(1), 55-60.

 9. Kim, Y.H., Sugiyama, M., Yamagishi, K., Kaneko, Y., Fukui, K., Kobayashi, A., and Harashima, S.
   (2005) A versatile and general splitting technology for generating targeted YAC subclones. Appl.
   Microbiol. Biotechnol., 69(1), 65-70.

 10. Harashima, S., Kaneko, Y., Sugiyama, M., Kim, Y.H. (2006) 새로운 시기에 들어간 효모연구의 최전선,
   Research Journal of Food and Agriculture (ISSN 0387-9240), 29(6), 5-10.

 11. Kim, Y.H., Sugiyama, M., Kaneko, Y., Fukui, K., Kobayashi, A., and Harashima, S. (2006) A
   PCR-mediated YAC splitting technology for generating targeted YAC subclones. Methods in
   Molecular Biology, vol.349 :YAC protocol 2nd edition (ISBN 1-59745-158-4), 103-115.

 12. Kim, Y.H., Ishikawa, D., Ha, H.P., Kaneko, Y., and Harashima, S. (2006) Chromosome XII
   structure is important for rDNA function in yeast. Nucleic Acids Research, 34(10), 2914-2924.

 13. E.J., Shin, S.L., Park, S.J., Jeon, J.W., Lee, Y.T., Kim, Y.H., Kim and S.W., Nam. (2006) Effect of
   molecular chaperones on the soluble expression of alginate lyase in E. coli. Biotechnology and    Bioprocess Engneering, 11, 414-419

 14. H.J., Kim, J.H., Lee, H.C., Kim, Y.H., Kim, H.J., Kwon, B.W., Kim and S.W., Nam. (2007) Cell
   surface display of cycloinulooligosaccharide fructanotransferase gene in Saccharomyces
   cerevisiae. Kor. J. Life Science, 17(2), 241-247.

 15. M.J., Kim, Y.H., Jang, M.H., Sung, Y.H., Kim and S.W., Nam. (2007) Constitutive expression of
   arylsulfatase from Pseudoalteromonas carrageenovora in E.coli and its application to preparation
   of agarose. Kor. J. Microbiol. Biotechnol., 35(1), 11-16.

 16. H.J., Kim, J.H., Lee, H.C., Kim, J.W., Lee, Y.H., Kim and S.W., Nam. (2007) Characterization of
   cyclofructans from inulin by Saccharomyces cerevisiae strain displaying cell-surface cycloinulooligo
   saccharide fructanotransferase. Journal of Microbiology and Biotechnology, 17(4), 695-700.

 17. M.Y., Lim, J.W., Lee, J.H., Lee, Y.H., Kim, J.H., Seo and S.W., Nam. (2007) Secretory
   overexpression of Clostridium endoglucanase A in Saccharomyces cerevisiae using GAL10
   promoter and exoinulinase signal sequence. Kor. J. Life Science, 17(9), 1248-1254.

 18. J.H., Lee, M.Y., Lim, M.J., Kim, S.Y., Heo, J.H., Seo., Y.H., Kim and S.W., Nam. (2007)
   Constitutive coexpression of Bacillus endoxylanase and Trichoderma endoglucanase genes in
   Saccharomyces cerevisiae. Journal of Microbiology and Biotechnology, 17(12), 2076-2080.

 19. S.H. Kim, B.H. Shin, Y.H. Kim, S.W. Nam, and S.J. Jeon. (2007) Cloning and expression of a
   full-length glutamate decarboxylase gene from Lactobacillus brevis BH2. Biotechnology and
   Bioprocess Engineering, 12, 707-712.

 20. Y.H. Kim, S.Y. Heo, M.J. Kim, J.H. Lee, Y.M. Kim, and S.W. Nam. (2008) Optimal production
   of xylooligosaccharide by using recombinant endoxylanase from Bacillus subtilis. Kor. J. Life
   Science, 18(1), 52-57.

 21. M.J. Kim, M.J. Kim, J.H. Lee, Y.H. Kim, J.H. Seo, and S.W. Nam. (2008) Secretory
   overexpression and charcterization of human procarboxypeptidase B from saccharomyces
   cerevisiae. Kor. J. Microbiol. Biotechnol. 36(1), 49-54.